Lipoxygenase Gene Expression in the Tobacco-Phytophthora parasitica nicotianae Interaction

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Lipoxygenase gene expression in the tobacco-Phytophthora parasitica nicotianae interaction.

A recently isolated cDNA clone of tobacco (Nicotiana tabacum L.) lipoxygenase (LOX) was used to study LOX gene expression in tobacco cell-suspension cultures and intact plants in response to infection with Phytophthora parasitica nicotianae (Ppn). Southern blot analysis of tobacco DNA indicated that only a small number of LOX genes hybridize to this probe. These genes were not constitutively ex...

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The incompatible interaction between Phytophthora parasitica var. nicotianae race 0 and tobacco is suppressed in transgenic plants expressing antisense lipoxygenase sequences.

Nicotiana tabacum 46-8 cultivar displays an incompatible interaction with race 0 of Phytophthora parasitica var. nicotianae (Ppn), a fungal pathogen of most tobacco cultivars. At the plant level, incompatibility is characterized by the induction of lipoxygenase (LOX, EC = 1.13.11.12) activity and localized hypersensitive cell death before defense gene activation. To evaluate the involvement of ...

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Hydrogen peroxide yields during the incompatible interaction of tobacco suspension cells inoculated with Phytophthora nicotianae.

Rates of H(2)O(2) production by tobacco suspension cells inoculated with zoospores from compatible or incompatible races of the pathogen Phytophthora nicotianae were followed by direct measurement of oxygen evolution from culture supernatants following catalase addition. Rates of HO(2)(*)/O(2)(-) production were compared by following the formation of the formazan of sodium, 3'-[1-[phenylamino-c...

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Random amplified polymorphic DNA analysis of China isolates of Phytophthora parasitica var. nicotianae from different districts

China isolates of Phytophthora parasitica var. Nicotianae have been divided into three large pathogenic groups (PGS) (Hv(A), Iv(B), Wv(C)) which came from Yunnan, Shandong and Liaoning. These three districts belong to main tobacco regions in China: 15 isolates derived from Yunnan of the southwest, 13 isolates from Shandong of the east and 20 isolates from Liaoning of the northeast. PGS were det...

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Na+/H+ exchanger 1 participates in tobacco disease defence against Phytophthora parasitica var. nicotianae by affecting vacuolar pH and priming the antioxidative system

Despite the importance of NHX1 (Na(+)/H(+) exchanger 1) in plant salt tolerance, little is known about its other functions. In this study, intriguingly, it was found that NHX1 participated in plant disease defence against Phytophthora parasitica var. nicotianae (Ppn) in Nicotiana benthamiana. NbNHX1 was originally isolated from N. benthamiana, and characterized. The subcellular localization of ...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1996

ISSN: 1532-2548,0032-0889

DOI: 10.1104/pp.112.3.997